reference non entero toxigenic ntbf strains atcc 25285 Search Results


bacteroides fragilis  (ATCC)
97
ATCC bacteroides fragilis
F3 and F4 fractions inhibited Salmonella translocation in vitro . (A) Effect of fractions (F1, F2a, F2b, F3, F4, and F5) on Salmonella Heildelberg (SH) growth [colony forming unit (CFU)/ml] after 3 h of infection. (B) Impact of <t>Bacteroides</t> <t>fragilis</t> F3, F4, or F5 fractions and supernatant (SN) on Salmonella Heidelberg translocation across an in vitro multicellular model mimicking the intestinal epithelium. Results were expressed as % of translocation normalized to Salmonella alone (SH). (C) Impact of SN, F3, F4, and F5 on SipA expression, (D) Impact of SN, F3, F4, and F5 on fliC expression. Results were normalized to the cells treated only with Salmonella . The Shapiro and the Kruskall-Wallis tests were performed on n = 5 for this experiment. The Shapiro test was used to verify normality; the one-way ANOVA test was performed on n = 5. All data are presented as means (SEM); * p < 0.05, ** p < 0.01.
Bacteroides Fragilis, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference non entero toxigenic ntbf strains atcc 25285  (ATCC)
99
ATCC reference non entero toxigenic ntbf strains atcc 25285
F3 and F4 fractions inhibited Salmonella translocation in vitro . (A) Effect of fractions (F1, F2a, F2b, F3, F4, and F5) on Salmonella Heildelberg (SH) growth [colony forming unit (CFU)/ml] after 3 h of infection. (B) Impact of <t>Bacteroides</t> <t>fragilis</t> F3, F4, or F5 fractions and supernatant (SN) on Salmonella Heidelberg translocation across an in vitro multicellular model mimicking the intestinal epithelium. Results were expressed as % of translocation normalized to Salmonella alone (SH). (C) Impact of SN, F3, F4, and F5 on SipA expression, (D) Impact of SN, F3, F4, and F5 on fliC expression. Results were normalized to the cells treated only with Salmonella . The Shapiro and the Kruskall-Wallis tests were performed on n = 5 for this experiment. The Shapiro test was used to verify normality; the one-way ANOVA test was performed on n = 5. All data are presented as means (SEM); * p < 0.05, ** p < 0.01.
Reference Non Entero Toxigenic Ntbf Strains Atcc 25285, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+non+entero+toxigenic+ntbf+strains+atcc+25285/Bacteroides+fragilis+(Veillon+and+Zuber)+Castellani+and+Chalmers/pmc07172567-2670-13-17
Average 99 stars, based on 1 article reviews
reference non entero toxigenic ntbf strains atcc 25285 - by Bioz Stars, 2026-10
99/100 stars
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F3 and F4 fractions inhibited Salmonella translocation in vitro . (A) Effect of fractions (F1, F2a, F2b, F3, F4, and F5) on Salmonella Heildelberg (SH) growth [colony forming unit (CFU)/ml] after 3 h of infection. (B) Impact of Bacteroides fragilis F3, F4, or F5 fractions and supernatant (SN) on Salmonella Heidelberg translocation across an in vitro multicellular model mimicking the intestinal epithelium. Results were expressed as % of translocation normalized to Salmonella alone (SH). (C) Impact of SN, F3, F4, and F5 on SipA expression, (D) Impact of SN, F3, F4, and F5 on fliC expression. Results were normalized to the cells treated only with Salmonella . The Shapiro and the Kruskall-Wallis tests were performed on n = 5 for this experiment. The Shapiro test was used to verify normality; the one-way ANOVA test was performed on n = 5. All data are presented as means (SEM); * p < 0.05, ** p < 0.01.

Journal: Frontiers in Microbiology

Article Title: Bacteroides fragilis derived metabolites, identified by molecular networking, decrease Salmonella virulence in mice model

doi: 10.3389/fmicb.2022.1023315

Figure Lengend Snippet: F3 and F4 fractions inhibited Salmonella translocation in vitro . (A) Effect of fractions (F1, F2a, F2b, F3, F4, and F5) on Salmonella Heildelberg (SH) growth [colony forming unit (CFU)/ml] after 3 h of infection. (B) Impact of Bacteroides fragilis F3, F4, or F5 fractions and supernatant (SN) on Salmonella Heidelberg translocation across an in vitro multicellular model mimicking the intestinal epithelium. Results were expressed as % of translocation normalized to Salmonella alone (SH). (C) Impact of SN, F3, F4, and F5 on SipA expression, (D) Impact of SN, F3, F4, and F5 on fliC expression. Results were normalized to the cells treated only with Salmonella . The Shapiro and the Kruskall-Wallis tests were performed on n = 5 for this experiment. The Shapiro test was used to verify normality; the one-way ANOVA test was performed on n = 5. All data are presented as means (SEM); * p < 0.05, ** p < 0.01.

Article Snippet: Bacteroides fragilis ( B. fragilis ) NTBF (Non Toxigenic Bacteroides fragilis ), ATCC 25285 ( ; ), was purchased from the American Type Culture Collection.

Techniques: Translocation Assay, In Vitro, Infection, Expressing

GNPS molecular networking of negative ion MS/MS spectral data showing a selection of ions in bioactive fractions (F3 and F4). (A) Data were recorded in negative ionization mode. The nodes display the measured average masses of the molecular ions with identical MS/MS spectra. The numbers on the nodes represent the parent mass of the molecules. Node size reflects the bioactivity scores of the ions; node color indicates the abundance in the different conditions. The nodes in orange correspond to compounds present both in B. fragilis supernatant and in F3; nodes in pink correspond to compounds present both in supernatant and F4; nodes in green correspond to compounds present in all (supernatant, F3 and F4); nodes in purple correspond to ions solely found in DMEM. GNPS analysis of B. fragilis supernatant and bioactive fractions after LC–MS/MS showed the presence of 24 specific molecules of these fractions, 11 molecules for F3, 10 molecules for F4, and 3 molecules for both. (B) Among them, cholic acid (CA) and deoxycholic acid were identified.

Journal: Frontiers in Microbiology

Article Title: Bacteroides fragilis derived metabolites, identified by molecular networking, decrease Salmonella virulence in mice model

doi: 10.3389/fmicb.2022.1023315

Figure Lengend Snippet: GNPS molecular networking of negative ion MS/MS spectral data showing a selection of ions in bioactive fractions (F3 and F4). (A) Data were recorded in negative ionization mode. The nodes display the measured average masses of the molecular ions with identical MS/MS spectra. The numbers on the nodes represent the parent mass of the molecules. Node size reflects the bioactivity scores of the ions; node color indicates the abundance in the different conditions. The nodes in orange correspond to compounds present both in B. fragilis supernatant and in F3; nodes in pink correspond to compounds present both in supernatant and F4; nodes in green correspond to compounds present in all (supernatant, F3 and F4); nodes in purple correspond to ions solely found in DMEM. GNPS analysis of B. fragilis supernatant and bioactive fractions after LC–MS/MS showed the presence of 24 specific molecules of these fractions, 11 molecules for F3, 10 molecules for F4, and 3 molecules for both. (B) Among them, cholic acid (CA) and deoxycholic acid were identified.

Article Snippet: Bacteroides fragilis ( B. fragilis ) NTBF (Non Toxigenic Bacteroides fragilis ), ATCC 25285 ( ; ), was purchased from the American Type Culture Collection.

Techniques: Tandem Mass Spectroscopy, Selection, Liquid Chromatography with Mass Spectroscopy

Cholic acid (CA) quantification in supernatant (SN) and in bioactive fractions (F3, F4) after HPLC-ELSD (High-performance liquid chromatography- evaporative light scattering detector) analysis.

Journal: Frontiers in Microbiology

Article Title: Bacteroides fragilis derived metabolites, identified by molecular networking, decrease Salmonella virulence in mice model

doi: 10.3389/fmicb.2022.1023315

Figure Lengend Snippet: Cholic acid (CA) quantification in supernatant (SN) and in bioactive fractions (F3, F4) after HPLC-ELSD (High-performance liquid chromatography- evaporative light scattering detector) analysis.

Article Snippet: Bacteroides fragilis ( B. fragilis ) NTBF (Non Toxigenic Bacteroides fragilis ), ATCC 25285 ( ; ), was purchased from the American Type Culture Collection.

Techniques: High Performance Liquid Chromatography